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Xia Jiahui
Strategic Study of CAE 2000, Volume 2, Issue 11, Pages 1-11
Keywords: genetic disease gene mapping and cloning gene familiy-candidate disease gene cloning genome wide scan
Factors affecting early embryonic development in cattle: relevance for bovine cloning
Yanna DANG, Kun ZHANG
Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 33-41 doi: 10.15302/J-FASE-2018228
Keywords: bovine cloning embryo development somatic cell nuclear transfer X-inactive specific transcript
Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao
Strategic Study of CAE 2014, Volume 16, Issue 9, Pages 50-60
The full length cDNA of LH has been cloned from pituitary of half smooth tongue sole C. Günther by using RACE technology for the first time. The cDNA of LH is 670 bp,and the open reading frame is 477 bp encoding peptides of 158 amino acid. Multiple amino acid sequences alignments were performed among vertebrates,and the results showed that tongue sole LH mature peptide was the most similar to their orthologs of Pleuronectiformes and Perciformes which was 58 %~68 % identity. And 12 conserved cysteine residues and 1 N-linked glycosylation site were found in the deduced amino acid sequence. Real-time PCR analysis showed that LH mRNA was expressed in all twelve tissues including brain,pituitary,ovary and so on. The highest expression level was in pituitary. And the stronger amplification signals were detected in the brain and gonad than all other non- pituitary tissues. It suggested that LH may had the more physiological functions because LH mRNA of tongue sole expressed widely in non-pituitary tissues. Quantitative real- time PCR was used for analyzing the expression of female C. semilaevis LH mRNA in the reproductive cycle. The results showed that LH mRNA was expressed in all three tissues including brain,pituitary and ovary from the stage Ⅱto stage Ⅵ, and the highest expression level appeared on the stage Ⅳ and stage Ⅴwhich suggested that the main function of LH is to promote eggs finally mature and discharge. Radio immunoassay (RIA)marked by 125I was used to detect the concentration of plasma LH in C.semilaevis. The results showed that the biggest plasma LH concentration appareed on the stage of Ⅴwhich also suggested that the main function of LH was to promote eggs finally mature and discharge.
Keywords: Cynoglossus semilaevis Günther LH cDNA cloning Quantitative real-time PCR RIA
Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning Article
Mingzhu Sun, Yaowei Liu, Maosheng Cui, Qili Zhao, Xiangfei Zhao, Yidi Zhang, Jingjing Huang, Guizhang Lu, Xin Zhao
Engineering 2023, Volume 24, Issue 5, Pages 73-83 doi: 10.1016/j.eng.2022.04.016
Since the first cloned sheep was produced in 1996, cloning has attracted considerable attention becauseThis low success rate is considered to be the major limitation in the extensive application of cloningWe then developed a robotic batch SCNT system to apply this micromanipulation method to animal cloningThe success rate of cloning was significantly increased compared to that of traditional methods (2.5%In addition to the cloning technique, the intracellular strain evaluation-based enucleation method is
Keywords: Oocyte enucleation Robotic cloning Intracellular strain Intracellular damage Optical flow
Effects of enucleation method on
Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI
Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 61-65 doi: 10.15302/J-FASE-2018227
Keywords: cloning enucleation pig SCNT
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 148-160 doi: 10.15302/J-FASE-2019305
Keywords: animal breeding cattle cloning CRISPR/Cas9 cytoplasmic injection embryo genome editing germline chimaeras
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 148-152 doi: 10.1007/s11684-009-0032-7
Keywords: gene X-linked inhibitor of apoptosis protein associated factor-1 (XAF1) promoter transcription regulation
cDNA Cloning of Neuropeptide Y gene of turbot Scophthalmus maximus L. and its expression analysis of
Han Jian,Liu Bin,Jiang Zhiqiang,Mu Xiaosheng,Lei Jilin
Strategic Study of CAE 2014, Volume 16, Issue 9, Pages 42-49
In this study, the full length of turbot NPY cDNA was cloned using RT-PCR and rapid-amplification of cDNA ends (RACE). Turbot NPY cDNA is 729bp with a 5´-untranslated region (UTR), 135bp 3´-UTR and 299bp ORF. The open reading frame encodes a 99 amino acid NPY. The first 28 amino acids constitute the signal sequence followed by the 36 amino acid mature peptide. Turbot NPY share a 53.9–98.0% identity with NPY from other fish, especially, Turbot NPY share a 97–98.0% identity with NPY from other flounder (Japanese flounder, winter flounder).Using real-time PCR to analysis NPY expression in turbot. Tissue distribution studies show that NPY mRNA is expressed in brain, in particular the hypothalamic, and in peripheral tissues. During food deprivation, the NPY undergo significant change in expression (P<0.05), with NPY levels being elevated first, then decreased, and being significantly increased, maintain a high level of expression, then decreased again during food deprivation. Expression level was significantly higher with refeeding after fasting than the normal feeding, the longer of food deprivation, the higher of expression level. But the expression level of 2h after refeeding will fall again to a similar level. Our results suggest that NPY is conserved peptides that might be involved in the regulation of feeding and other physiological functions in turbot.
Keywords: Turbot (Scophthalmus maximus L.) Neuropeptide Y Starvation Feeding Differential expression
Factors influencing the somatic cell nuclear transfer efficiency in pigs
Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI
Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 73-80 doi: 10.15302/J-FASE-2018220
Using a data set from our laboratory, we assessed the effects of several factors on pig cloning efficiencyconfluence (>90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall cloningof blastocysts transferred by somatic cell nuclear transfer (SCNT) did not significantly affect the cloning
Keywords: blastocyst donor cell estrus synchronization pregnancy rate pig cloning somatic cell nuclear transfer
Title Author Date Type Operation
Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases
Xia Jiahui
Journal Article
Factors affecting early embryonic development in cattle: relevance for bovine cloning
Yanna DANG, Kun ZHANG
Journal Article
Full length cDNA cloning and expression of luteinizing hormone (LH) and which serum concentration was
Liu Xuezhou,Shi Bao,Wang Shanshan,Xu Yongjiang,Li Xiaoxiao
Journal Article
Intracellular Strain Evaluation-Based Oocyte Enucleation and Its Application in Robotic Cloning
Mingzhu Sun, Yaowei Liu, Maosheng Cui, Qili Zhao, Xiangfei Zhao, Yidi Zhang, Jingjing Huang, Guizhang Lu, Xin Zhao
Journal Article
Effects of enucleation method on
Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI
Journal Article
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
Journal Article
Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
Journal Article
cDNA Cloning of Neuropeptide Y gene of turbot Scophthalmus maximus L. and its expression analysis of
Han Jian,Liu Bin,Jiang Zhiqiang,Mu Xiaosheng,Lei Jilin
Journal Article
Factors influencing the somatic cell nuclear transfer efficiency in pigs
Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI
Journal Article