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PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing Article

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Engineering 2022, Volume 15, Issue 8,   Pages 67-77 doi: 10.1016/j.eng.2022.02.013

Abstract: CRISPR associated protein 9 (St1Cas9) cytosine base editor (CBE)—but not the widely usedStreptococcus pyogenes Cas9 (SpCas9) or Lachnospiraceae bacterium Cpf1 (LbCpf1) CBEsDespite the notable C-to-T conversions, a high proportion of undesired byproducts exists with St1Cas9We therefore engineered St1Cas9 CBE by means of uracil DNA glycosylase inhibitor (UGI) or uracil DNABecause wild-type St1Cas9 recognizes a relatively strict protospacer adjacent motif (PAM) sequence for

Keywords： CRISPR     Cas9     Mycobacterium tuberculosis     Genome editing     Base editing    

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter of mice using the CRISPR/Cas9 system to reduce or eliminate lactoferrin expression.(7/29) by injecting the plasmid pX330, expressing a small guide RNA and human codon-optimized SpCas9,

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 2-5 doi: 10.15302/J-FASE-2014007

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Mobile CRISPR-Cas9 based anti-phage system in E. coli

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 8,   Pages 1281-1289 doi: 10.1007/s11705-022-2141-7

Abstract: Here, we developed a mobile CRISPR-Cas9 based anti-phage system for bacteriophages defense in E. coli

Keywords： phage infections     anti-phage     CRISPR-Cas9     conjugative transfer     synthetic biology    

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CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 554-562 doi: 10.1007/s11684-017-0543-6

Abstract: successfully generated LAG-3 knockout T and CAR-T cells with high efficiency using CRISPR-Cas9

Keywords： CAR-T     CRISPR-Cas9     LAG-3    

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Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 148-160 doi: 10.15302/J-FASE-2019305

Abstract:

Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.

Keywords： animal breeding     cattle     cloning     CRISPR/Cas9     cytoplasmic injection     embryo     genome editing     germline chimaeras    

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Disease-Resistant Channel Catfish (Ictalurus punctatus) Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering Article

Jinhai Wang,Baofeng Su,De Xing,Timothy J. Bruce,Shangjia Li,Logan Bern,Mei Shang,Andrew Johnson,Rhoda Mae C. Simora,Michael Coogan,Darshika U. Hettiarachchi,Wenwen Wang,Tasnuba Hasin,Jacob Al-Armanazi,Cuiyu Lu,Rex A. Dunham

Engineering doi: 10.1016/j.eng.2023.12.005

Abstract: versatile tool for genome manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) platform holds promise for modifying fish traits of interest.Taking advantage of the CRISPR/Cas9-mediated system, we succeeded in integrating the cathelicidin gene

Keywords： Genome editing     ssODN     dsDNA     Antimicrobial peptide     Reproductive confinement     Aquaculture    

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is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 690-704 doi: 10.1007/s11684-018-0638-8

Abstract: in hearing, we generated -null mice by clustered regularly interspaced short palindromic repeat/Cas9

Keywords： TPRN     stereocilia     stereociliary rootlet     actin filament     CRISPR/Cas9     hearing    

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Transcription Factors HNF1A, HNF4A, and FOXA2 Regulate Hepatic Cell Protein N-Glycosylation Article

Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš

Engineering 2024, Volume 32, Issue 1,   Pages 57-68 doi: 10.1016/j.eng.2023.09.019

Abstract: We used the state-of-the-art clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) molecular tool for the downregulation of the HNF1A, HNF4A, and FOXA2

Keywords： Clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9)     Epigenetics     Hepatocyte    

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Genome-edited crops: how to move them from laboratory to market

Kunling CHEN, Caixia GAO

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 181-187 doi: 10.15302/J-FASE-2020332

Abstract:

Recent breakthroughs in CRISPR technology allow specific genome manipulation of almost all crops and

Keywords： CRISPR/Cas     genome editing     base editing     precision breeding     regulation    

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First CRISPR-Based Therapy Poised to Reach Patients

Jennifer Welsh

Engineering 2023, Volume 30, Issue 11,   Pages 3-6 doi: 10.1016/j.eng.2023.09.005

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Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal

Xiaoran Sun, Jiagui Song, Jing Zhang, Jun Zhan, Weigang Fang, Hongquan Zhang

Frontiers of Medicine 2020, Volume 14, Issue 1,   Pages 91-100 doi: 10.1007/s11684-019-0696-6

Abstract: AcK27-HOXB9 is a newly identified HOXB9 post-transcriptional modification that can predict the outcomeHOXB9 was upregulated ( <0.0001), and AcK27-HOXB9 ( =0.0023) was downregulated in patients with PDAC.HOXB9 promoted ( =0.0115), while AcK27-HOXB9 ( =0.0279) inhibited PDAC progression.The results of this study showed that HOXB9 promoted and AcK27-HOXB9 suppressed PDAC progression.The determination of ratio between HOXB9 and AcK27-HOXB9 exhibited potential diagnostic value in patients

Keywords： HOXB9     AcK27-HOXB9     PDAC    

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662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)

QU Yanchun, YANG Ze, SUN Liang, JI Linong

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 283-285 doi: 10.1007/s11684-008-0053-7

Abstract: The aim of this paper is to report a new coding variance of the gene, a candidate for autoimmune diseases. We found the variation in two families with type 2 diabetes mellitus by D-HPLC mutation screening method and confirmed our results by direct sequencing and PCR-RFLP. Although without changing the amino acid coding, the variance may have an effect on codon usage and play a role in disease development, such as type 2 diabetes mellitus. However, we cannot define the role of this variance because the frequency of the minor allele is low in the Chinese population and no homozygote of the variance was found. More research in multiple populations will be necessary to define the role of this variance.

Keywords： D-HPLC mutation     development     autoimmune     PCR-RFLP     candidate    

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The 9th China Engineering Management Forum Guangzhou Consensus

Frontiers of Engineering Management 2015, Volume 2, Issue 2,   Pages 105-107 doi: 10.15302/J-FEM-2015019

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CRISPR Narrows Gap between Genetically Altered Foods and Consumers

Sarah C.P. Williams

Engineering 2023, Volume 20, Issue 1,   Pages 6-8 doi: 10.1016/j.eng.2022.11.002

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Title Author Date Type Operation

PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Journal Article

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Journal Article

Mobile CRISPR-Cas9 based anti-phage system in E. coli

Journal Article

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Journal Article

Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

Journal Article

Disease-Resistant Channel Catfish (Ictalurus punctatus) Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering

Jinhai Wang,Baofeng Su,De Xing,Timothy J. Bruce,Shangjia Li,Logan Bern,Mei Shang,Andrew Johnson,Rhoda Mae C. Simora,Michael Coogan,Darshika U. Hettiarachchi,Wenwen Wang,Tasnuba Hasin,Jacob Al-Armanazi,Cuiyu Lu,Rex A. Dunham

Journal Article

is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

Journal Article

Transcription Factors HNF1A, HNF4A, and FOXA2 Regulate Hepatic Cell Protein N-Glycosylation

Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš

Journal Article

Genome-edited crops: how to move them from laboratory to market

Kunling CHEN, Caixia GAO

Journal Article

First CRISPR-Based Therapy Poised to Reach Patients

Jennifer Welsh

Journal Article

Acetylated HOXB9 at lysine 27 is of differential diagnostic value in patients with pancreatic ductal

Xiaoran Sun, Jiagui Song, Jing Zhang, Jun Zhan, Weigang Fang, Hongquan Zhang

Journal Article

662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)

QU Yanchun, YANG Ze, SUN Liang, JI Linong

Journal Article

The 9th China Engineering Management Forum Guangzhou Consensus

Journal Article

CRISPR Narrows Gap between Genetically Altered Foods and Consumers

Sarah C.P. Williams

Journal Article