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First CRISPR-Based Therapy Poised to Reach Patients

Jennifer Welsh

Engineering 2023, Volume 30, Issue 11,   Pages 3-6 doi: 10.1016/j.eng.2023.09.005

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Mobile CRISPR-Cas9 based anti-phage system in E. coli

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 8,   Pages 1281-1289 doi: 10.1007/s11705-022-2141-7

Abstract: Here, we developed a mobile CRISPR-Cas9 based anti-phage system for bacteriophages defense in E. coli

Keywords： phage infections     anti-phage     CRISPR-Cas9     conjugative transfer     synthetic biology    

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CRISPR Narrows Gap between Genetically Altered Foods and Consumers

Sarah C.P. Williams

Engineering 2023, Volume 20, Issue 1,   Pages 6-8 doi: 10.1016/j.eng.2022.11.002

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter of mice using the CRISPR

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Genome reprogramming for synthetic biology

Kylie Standage-Beier,Xiao Wang

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 37-45 doi: 10.1007/s11705-017-1618-2

Abstract: The ability to go from a digitized DNA sequence to a predictable biological function is central to synthetic biology. Genome engineering tools facilitate rewriting and implementation of engineered DNA sequences. Recent development of new programmable tools to reengineer genomes has spurred myriad advances in synthetic biology. Tools such as clustered regularly interspace short palindromic repeats enable RNA-guided rational redesign of organisms and implementation of synthetic gene systems. New directed evolution methods generate organisms with radically restructured genomes. These restructured organisms have useful new phenotypes for biotechnology, such as bacteriophage resistance and increased genetic stability. Advanced DNA synthesis and assembly methods have also enabled the construction of fully synthetic organisms, such as J. Craig Venter Institute (JCVI)-syn 3.0. Here we summarize the recent advances in programmable genome engineering tools.

Keywords： CRISPR     genome engineering     synthetic biology     rational design    

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CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 554-562 doi: 10.1007/s11684-017-0543-6

Abstract: successfully generated LAG-3 knockout T and CAR-T cells with high efficiency using CRISPR-Cas9

Keywords： CAR-T     CRISPR-Cas9     LAG-3    

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CRISPR Use in Humans Shows Promise While Experts Weigh Ethical Concerns

Peter Weiss

Engineering 2020, Volume 6, Issue 7,   Pages 719-722 doi: 10.1016/j.eng.2020.05.010

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Advances in genetic engineering of domestic animals

Shaohua WANG,Kun ZHANG,Yunping DAI

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 1-10 doi: 10.15302/J-FASE-2016085

Abstract: Global population will increase to over nine billion by 2050 with the doubling in demand for meat and milk. To overcome this challenge, it is necessary to breed highly efficient and productive livestock. Furthermore, livestock are also excellent models for human diseases and ideal bioreactors to produce pharmaceutical proteins. Thus, genetic engineering of domestic animals presents a critical and valuable tool to address these agricultural and biomedical applications. Overall, genetic engineering has evolved through three stages in history: transgenesis, gene targeting, and gene editing. Since the birth of the first transgenic pig, genetic engineering in livestock has been advancing slowly due to inherent technical limitations. A major breakthrough has been the advent of somatic cell nuclear transfer, which, for the first time, provided the technical ability to produce site-specific genome-modified domestic animals. However, the low efficiency of gene targeting events in somatic cells prohibits its wide use in agricultural and biomedical applications. Recently, rapid progress in tools and methods of genome engineering has been made, allowing genetic editing from mutation of a single base pair to the deletion of entire chromosomes. Here, we review the major advances of genetic engineering in domestic animals with emphasis placed on the introduction of latest designer nucleases.

Keywords： CRISPR     TALEN     ZFN     gene editing     livestock     pig     cattle    

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One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1,   Pages 2-5 doi: 10.15302/J-FASE-2014007

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Livestock breeding for the 21st century: the promise of the editing revolution

Chris PROUDFOOT, Gus MCFARLANE, Bruce WHITELAW, Simon LILLICO

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 129-135 doi: 10.15302/J-FASE-2019304

Abstract:

In recent years there has been a veritable explosion in the use of genome editors to create site-specific changes, both and , to the genomes of a multitude of species for both basic research and biotechnology. Livestock, which form a vital component of most societies, are no exception. While selective breeding has been hugely successful at enhancing some production traits, the rate of progress is often slow and is limited to variants that exist within the breeding population. Genome editing provides the potential to move traits between breeds, in a single generation, with no impact on existing productivity or to develop phenotypes that tackle intractable issues such as disease. As such, genome editors provide huge potential for ongoing livestock development programs in light of increased demand and disease challenge. This review will highlight some of the more notable agricultural applications of this technology in livestock.

Keywords： cattle     pig     sheep     chicken     aquaculture     CRISPR    

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PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing in Mycobacteria Article

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Engineering 2022, Volume 15, Issue 8,   Pages 67-77 doi: 10.1016/j.eng.2022.02.013

Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR) base editors allow for rapid, robustBy screening distinct CRISPR base editors, we discovered that only the unusual Streptococcus thermophilus CRISPR associated protein 9 (St1Cas9) cytosine base editor (CBE)—but not the widely used

Keywords： CRISPR     Cas9     Mycobacterium tuberculosis     Genome editing     Base editing    

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Genome-edited crops: how to move them from laboratory to market

Kunling CHEN, Caixia GAO

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 181-187 doi: 10.15302/J-FASE-2020332

Abstract:

Recent breakthroughs in CRISPR technology allow specific genome manipulation of almost all crops and

Keywords： CRISPR/Cas     genome editing     base editing     precision breeding     regulation    

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A brief review of genome editing technology for generating animal models

Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 123-128 doi: 10.15302/J-FASE-2019309

Abstract:

The recent development of genome editing technologies has given researchers unprecedented power to alter DNA sequences at chosen genomic loci, thereby generating various genetically edited animal models. This mini-review briefly summarizes the development of major genome editing tools, focusing on the application of these tools to generate animal models in multiple species.

Keywords： animal model     CRISPR     genome editing     TALEN     ZFN    

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Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 148-160 doi: 10.15302/J-FASE-2019305

Abstract:

Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.

Keywords： animal breeding     cattle     cloning     CRISPR/Cas9     cytoplasmic injection     embryo     genome editing     germline chimaeras    

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is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 690-704 doi: 10.1007/s11684-018-0638-8

Abstract: encodes the taperin protein, which is concentrated in the tapered region of hair cell stereocilia in the inner ear. In humans, mutations cause autosomal recessive nonsyndromic deafness (DFNB79) by an unknown mechanism. To determine the role of in hearing, we generated -null mice by clustered regularly interspaced short palindromic repeat/Cas9 genome-editing technology from a CBA/CaJ background. We observed significant hearing loss and progressive degeneration of stereocilia in the outer hair cells of -null mice starting from postnatal day 30. Transmission electron microscopy images of stereociliary bundles in the mutant mice showed some stereociliary rootlets with curved shafts. The central cores of the stereociliary rootlets possessed hollow structures with surrounding loose peripheral dense rings. Radixin, a protein expressed at stereocilia tapering, was abnormally dispersed along the stereocilia shafts in null mice. The expression levels of radixin and -actin significantly decreased. We propose that is critical to the retention of the integrity of the stereociliary rootlet. Loss of in -null mice caused the disruption of the stereociliary rootlet, which resulted in damage to stereociliary bundles and hearing impairments. The generated -null mice are ideal models of human hereditary deafness DFNB79.

Keywords： TPRN     stereocilia     stereociliary rootlet     actin filament     CRISPR/Cas9     hearing    

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Title Author Date Type Operation

First CRISPR-Based Therapy Poised to Reach Patients

Jennifer Welsh

Journal Article

Mobile CRISPR-Cas9 based anti-phage system in E. coli

Journal Article

CRISPR Narrows Gap between Genetically Altered Foods and Consumers

Sarah C.P. Williams

Journal Article

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

Genome reprogramming for synthetic biology

Kylie Standage-Beier,Xiao Wang

Journal Article

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Journal Article

CRISPR Use in Humans Shows Promise While Experts Weigh Ethical Concerns

Peter Weiss

Journal Article

Advances in genetic engineering of domestic animals

Shaohua WANG,Kun ZHANG,Yunping DAI

Journal Article

One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system

Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN

Journal Article

Livestock breeding for the 21st century: the promise of the editing revolution

Chris PROUDFOOT, Gus MCFARLANE, Bruce WHITELAW, Simon LILLICO

Journal Article

PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing in Mycobacteria

Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji

Journal Article

Genome-edited crops: how to move them from laboratory to market

Kunling CHEN, Caixia GAO

Journal Article

A brief review of genome editing technology for generating animal models

Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH

Journal Article

Embryo-mediated genome editing for accelerated genetic improvement of livestock

Zachariah MCLEAN, Björn OBACK, Götz LAIBLE

Journal Article

is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao

Journal Article