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八步连续流全合成维生素B1工艺研究 Article
姜梅芬, 刘敏杰, 李伟剑, 夏应奇, 陈芬儿
《工程(英文)》 2024年 第32卷 第1期 页码 230-239 doi: 10.1016/j.eng.2023.01.016
维生素B1作为一种抗利尿和抗氧化剂被广泛应用于医疗保健和食品行业,以维持神经传导、心脏和胃肠道的正常功能。本研究报道了由市售2-氰基乙酰胺进行的维生素B1的八步连续流全合成工艺。维生素B1全合成的连续流工艺总停留时间约为3.5小时,产品纯度高,分离产率为47.7%。该八步连续流动技术方案至少涉及绿色化学的六个关键准则。
Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice
null
《医学前沿(英文)》 2016年 第10卷 第4期 页码 481-489 doi: 10.1007/s11684-016-0494-3
The tumor suppressor gene liver kinase B1 (LKB1), also called STK11, encodes a serine/threonine kinase. LKB1 plays crucial roles in cell differentiation, proliferation, and polarity. In this study, LKB1 conditional knockout mice (LKB1Pax2 CKO mice) were generated using Pax2-Cre mice to investigate the function of LKB1 in inner ear hair cells during early embryonic period. LKB1Pax2 CKO mice died perinatally. Immunofluorescence and scanning electron microscopy revealed that stereociliary bundles in LKB1Pax2 CKO mice were clustered and misoriented, respectively. Moreover, ectopic distribution of kinocilium bundles resulting from abnormal migration of kinocilium was observed in the mutant mice. The orientation of stereociliary bundles and the migration of kinocilia are critical indicators of planar cell polarity (PCP) of hair cells. LKB1 deficiency in LKB1Pax2 CKO mice thus disrupted hair cell planar polarity during embryonic development. Our results suggest that LKB1 is required in PCP formation in cochlear hair cells in mice.
关键词: LKB1 stereociliary bundles kinocilium planar cell polarity hearing mice
LI Yanhua, BI Zhigang
《医学前沿(英文)》 2007年 第1卷 第1期 页码 79-86 doi: 10.1007/s11684-007-0016-4
基于数字信息素和领航算法的未知环境多智能体目标探测 Research
邵燕1,赵志峰1,2,李荣鹏1,周裕庚3
《信息与电子工程前沿(英文)》 2020年 第21卷 第5期 页码 649-808 doi: 10.1631/FITEE.1900659
关键词: 群体智能;数字信息素;人工势场;领航算法
《医学前沿(英文)》 2022年 第16卷 第6期 页码 883-895 doi: 10.1007/s11684-022-0919-0
关键词: osteosarcoma autophagy metastasis drug resistance Beclin1 LC3B
Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX
null
《医学前沿(英文)》 2016年 第10卷 第2期 页码 212-218 doi: 10.1007/s11684-016-0438-y
Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5×1011 and 1×1011 virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.
关键词: hemophilia B AAV8 hFIX gene therapy
null
《医学前沿(英文)》 2014年 第8卷 第2期 页码 217-226 doi: 10.1007/s11684-014-0326-2
This study was designed to investigate the contribution of miRNA-122-binding site polymorphism at the IL-1A gene and its multiplicative interactions with hepatitis B virus (HBV) mutations in the risk of hepatocellular carcinoma (HCC). A total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positive subjects (including 1021 HCC patients) were enrolled in this study. Quantitative PCR was used to genotype rs3783553. HBV mutations were determined by direct sequencing. Multivariate logistic regression analyses were performed to test the associations of rs3783553, mutations, and their interactions with the risk of HCC. No significant association was found between rs3783553 and the risk of HCC among healthy controls, HBsAg seroclearance subjects, HBsAg-positive subjects without HCC, and all controls. Additionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen seroconversion, abnormal alanine aminotransferase, and high viral load (>104 copies/ml). However, the TTCA insertion allele of rs3783553 was significantly associated with an increased frequency of HBV C7A mutation compared with homozygous TTCA deletion carriers [(del/ins+ ins/ins) vs. del/del, adjusted odds ratio (OR)=1.48, 95% confidence interval (CI)=1.09-2.02, P=0.013]. Multiplicative interaction of rs3783553 with HBV preS deletion significantly reduced the risk of HCC in males, with an adjusted OR of 0.64 (95% CI=0.42-0.98; P=0.041) after age and HBV genotype were adjusted. Although rs3783553 did not significantly affect genetic susceptibility to HBV-related HCC, its variant allele may predispose the host to selecting HBV C7A mutation during evolution and significantly reduce the risk of HCC caused by HBV preS deletion. This study provides an insight into the complex host-virus interaction in HBV-induced hepatocarcinogenesis and is helpful in determining HBsAg-positive subjects who are likely to develop HCC.
关键词: hepatocellular carcinoma (HCC) interaction miRNA-122-binding site IL-1A rs3783553 hepatitis B virus (HBV) mutations
Netrin-1 works with UNC5B to regulate angiogenesis in diabetic kidney disease
Xiaojing Jiao, Dong Zhang, Quan Hong, Lei Yan, Qiuxia Han, Fengmin Shao, Guangyan Cai, Xiangmei Chen, Hanyu Zhu
《医学前沿(英文)》 2020年 第14卷 第3期 页码 293-304 doi: 10.1007/s11684-019-0715-7
关键词: netrin-1 VEGF-165 UNC5B angiogenesis diabetic kidney disease
Molecular characterization of two suppressor of cytokine signaling 1 genes (
Xue XU,Jiannan ZHANG,Juan LI,Yajun WANG
《农业科学与工程前沿(英文)》 2015年 第2卷 第1期 页码 73-83 doi: 10.15302/J-FASE-2015044
左炔诺孕酮宫内释放系统对胰岛素样生长因子-1的影响与预防盆腔炎的相关性研究
吴晓杰,刘霞,陶跃平,王洁
《中国工程科学》 2015年 第17卷 第6期 页码 4-7
目的:研究左炔诺孕酮宫内释放系统对子宫内膜组织胰岛素样生长因子-1(IGF-1)的影响及预防盆腔炎疗效分析。分别对术前及术后6个月子宫内膜组织IGF-1的表达情况进行对比,且随访2年,了解患者盆腔炎发生情况。结果:所有手术均成功,研究组子宫内膜组织IGF-1表达术后明显低于术前,对照组术前及术后子宫内膜组织IGF-1表达变化无差异,二组相比,术后IGF-1表达差异有显著性。结论:左炔诺孕酮宫内释放系统对子宫内膜的IGF-1表达存在抑制作用,可能是其抑制子宫内膜增生并减少盆腔炎发生的机制之一。
基于双目立体匹配和B样条的三维人脸点云孔洞修补算法 Research Articles
黄源1,2,达飞鹏1,2
《信息与电子工程前沿(英文)》 2022年 第23卷 第3期 页码 398-408 doi: 10.1631/FITEE.2000508
关键词: 三维点云;孔洞修补;立体匹配;B样条
Irreversible phenotypic perturbation and functional impairment of B cells during HIV-1 infection
Jingjing Yan, Shuye Zhang, Jun Sun, Jianqing Xu, Xiaoyan Zhang
《医学前沿(英文)》 2019年 第13卷 第3期 页码 409-409 doi: 10.1007/s11684-018-0618-z
从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用 Article
Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee
《工程(英文)》 2024年 第32卷 第1期 页码 42-57 doi: 10.1016/j.eng.2023.08.001
A universal vaccine is in high demand to address the uncertainties of antigenic drift and the reduced effectiveness of current influenza vaccines. In this study, a strategy called computationally optimized broadly reactive antigen (COBRA) was used to generate a consensus sequence of the hemagglutinin globular head portion (HA1) of influenza virus samples collected from 1918 to 2021 to trace evolutionary changes and incorporate them into the designed constructs. Constructs carrying different HA1 regions were delivered into eukaryotic cells by Salmonella-mediated bactofection using a Semliki Forest virus RdRp-dependent eukaryotic expression system, pJHL204. Recombinant protein expression was confirmed by Western blot and immunofluorescence assays. Mice immunized with the designed constructs produced a humoral response, with a significant increase in immunoglobulin G (IgG) levels, and a cell-mediated immune response, including a 1.5-fold increase in CD4+ and CD8+ T cells. Specifically, constructs #1 and #5 increased the production of interferon-γ (IFN-γ) producing CD4+ and CD8+ T cells, skewing the response toward the T helper type 1 cell (Th1) pathway. Additionally, interleukin-4 (IL-4)-producing T cells were upregulated 4-fold. Protective efficacy was demonstrated, with up to 4-fold higher production of neutralizing antibodies and a hemagglutination inhibition titer > 40 against the selected viral strains. The designed constructs conferred a broadly protective immune response, resulting in a notable reduction in viral titer and minimal inflammation in the lungs of mice challenged with the influenza A/PR8/34, A/Brisbane/59/2007, A/California/07/2009, KBPV VR-92, and NCCP 43021 strains. This discovery revolutionizes influenza vaccine design and delivery; Salmonella-mediated COBRA-HA1 is a highly effective in vivo antigen presentation strategy. This approach can effectively combat seasonal H1N1 influenza strains and potential pandemic outbreaks.
关键词: COBRA Influenza A Salmonella Vaccine Broad spectral protection
Design, synthesis, and antiviral properties of 2-aryl-1H-benzimidazole-4-carboxamide derivatives
Xianjin LUO, Zhonglü ZHANG, Yutian YANG, Fei XUE, Naiyun XIU, Yuanbin SHE
《化学科学与工程前沿(英文)》 2009年 第3卷 第3期 页码 305-309 doi: 10.1007/s11705-009-0021-z
熊文成,申文明,王桥,史园莉,肖如林,付卓
《中国工程科学》 2014年 第16卷 第3期 页码 37-42
环境一号卫星A/B星是我国自主研制的小卫星星座,其在轨稳定运行近5年,并稳定获取了大量高质量的观测数据,实现对这些观测数据的几何精校正处理,对发挥环境星宏观、动态的生态环境监测有着重要意义。实际运行数据表明,该方法可以较好地实现环境一号卫星各载荷数据的自动几何精校正,环境一号卫星CCD相机自动几何精校正图像精度可达到2个像素以内,同星不同载荷图像之间自动匹配方法可以获取小于1个像素的图像匹配精度
标题 作者 时间 类型 操作
Ultraviolet-B induced expression of hypoxia-inducible factor 1α, transferrin receptor through EGFR/PI3K/AKT/DEC1 pathway
LI Yanhua, BI Zhigang
期刊论文
Superenhancers activate the autophagy-related genes Beclin1 and LC3B to drive metastasis and drug resistance
期刊论文
Association of miRNA-122-binding site polymorphism at the interleukin-1 α gene and its interaction withhepatitis B virus mutations with hepatocellular carcinoma risk
null
期刊论文
Netrin-1 works with UNC5B to regulate angiogenesis in diabetic kidney disease
Xiaojing Jiao, Dong Zhang, Quan Hong, Lei Yan, Qiuxia Han, Fengmin Shao, Guangyan Cai, Xiangmei Chen, Hanyu Zhu
期刊论文
Molecular characterization of two suppressor of cytokine signaling 1 genes (
Xue XU,Jiannan ZHANG,Juan LI,Yajun WANG
期刊论文
Irreversible phenotypic perturbation and functional impairment of B cells during HIV-1 infection
Jingjing Yan, Shuye Zhang, Jun Sun, Jianqing Xu, Xiaoyan Zhang
期刊论文
从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用
Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee
期刊论文
Design, synthesis, and antiviral properties of 2-aryl-1H-benzimidazole-4-carboxamide derivatives
Xianjin LUO, Zhonglü ZHANG, Yutian YANG, Fei XUE, Naiyun XIU, Yuanbin SHE
期刊论文