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First CRISPR-Based Therapy Poised to Reach Patients
Jennifer Welsh
Engineering 2023, Volume 30, Issue 11, Pages 3-6 doi: 10.1016/j.eng.2023.09.005
Mobile CRISPR-Cas9 based anti-phage system in E. coli
Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 8, Pages 1281-1289 doi: 10.1007/s11705-022-2141-7
Keywords: phage infections anti-phage CRISPR-Cas9 conjugative transfer synthetic biology
CRISPR Narrows Gap between Genetically Altered Foods and Consumers
Sarah C.P. Williams
Engineering 2023, Volume 20, Issue 1, Pages 6-8 doi: 10.1016/j.eng.2022.11.002
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3, Pages 242-248 doi: 10.15302/J-FASE-2015059
Keywords: lactoferrin promoter CRISPR/Cas9 plasmid pX330
Genome reprogramming for synthetic biology
Kylie Standage-Beier,Xiao Wang
Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1, Pages 37-45 doi: 10.1007/s11705-017-1618-2
Keywords: CRISPR genome engineering synthetic biology rational design
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang
Frontiers of Medicine 2017, Volume 11, Issue 4, Pages 554-562 doi: 10.1007/s11684-017-0543-6
Keywords: CAR-T CRISPR-Cas9 LAG-3
CRISPR Use in Humans Shows Promise While Experts Weigh Ethical Concerns
Peter Weiss
Engineering 2020, Volume 6, Issue 7, Pages 719-722 doi: 10.1016/j.eng.2020.05.010
Advances in genetic engineering of domestic animals
Shaohua WANG,Kun ZHANG,Yunping DAI
Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1, Pages 1-10 doi: 10.15302/J-FASE-2016085
Keywords: CRISPR TALEN ZFN gene editing livestock pig cattle
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 1, Pages 2-5 doi: 10.15302/J-FASE-2014007
Livestock breeding for the 21st century: the promise of the editing revolution
Chris PROUDFOOT, Gus MCFARLANE, Bruce WHITELAW, Simon LILLICO
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 129-135 doi: 10.15302/J-FASE-2019304
In recent years there has been a veritable explosion in the use of genome editors to create site-specific changes, both and , to the genomes of a multitude of species for both basic research and biotechnology. Livestock, which form a vital component of most societies, are no exception. While selective breeding has been hugely successful at enhancing some production traits, the rate of progress is often slow and is limited to variants that exist within the breeding population. Genome editing provides the potential to move traits between breeds, in a single generation, with no impact on existing productivity or to develop phenotypes that tackle intractable issues such as disease. As such, genome editors provide huge potential for ongoing livestock development programs in light of increased demand and disease challenge. This review will highlight some of the more notable agricultural applications of this technology in livestock.
Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji
Engineering 2022, Volume 15, Issue 8, Pages 67-77 doi: 10.1016/j.eng.2022.02.013
Keywords: CRISPR Cas9 Mycobacterium tuberculosis Genome editing Base editing
Genome-edited crops: how to move them from laboratory to market
Kunling CHEN, Caixia GAO
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 181-187 doi: 10.15302/J-FASE-2020332
Recent breakthroughs in CRISPR technology allow specific genome manipulation of almost all crops and
Keywords: CRISPR/Cas genome editing base editing precision breeding regulation
A brief review of genome editing technology for generating animal models
Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 123-128 doi: 10.15302/J-FASE-2019309
The recent development of genome editing technologies has given researchers unprecedented power to alter DNA sequences at chosen genomic loci, thereby generating various genetically edited animal models. This mini-review briefly summarizes the development of major genome editing tools, focusing on the application of these tools to generate animal models in multiple species.
Keywords: animal model CRISPR genome editing TALEN ZFN
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2, Pages 148-160 doi: 10.15302/J-FASE-2019305
Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.
Keywords: animal breeding cattle cloning CRISPR/Cas9 cytoplasmic injection embryo genome editing germline chimaeras
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
Frontiers of Medicine 2019, Volume 13, Issue 6, Pages 690-704 doi: 10.1007/s11684-018-0638-8
Keywords: TPRN stereocilia stereociliary rootlet actin filament CRISPR/Cas9 hearing
Title Author Date Type Operation
CRISPR Narrows Gap between Genetically Altered Foods and Consumers
Sarah C.P. Williams
Journal Article
Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330
Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG
Journal Article
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells
Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang
Journal Article
Advances in genetic engineering of domestic animals
Shaohua WANG,Kun ZHANG,Yunping DAI
Journal Article
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
Hongbing HAN,Yonghe MA,Tao WANG,Ling LIAN,Xiuzhi TIAN,Rui HU,Shoulong DENG,Kongpan LI,Feng WANG,Ning LI,Guoshi LIU,Yaofeng ZHAO,Zhengxing LIAN
Journal Article
Livestock breeding for the 21st century: the promise of the editing revolution
Chris PROUDFOOT, Gus MCFARLANE, Bruce WHITELAW, Simon LILLICO
Journal Article
PAM-Expanded Streptococcus thermophilus Cas9 C-to-T and C-to-G Base Editors for Programmable Base Editing in Mycobacteria
Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang, Weizhong Chen, Xia Zhang, Xingxu Huang, Wei Chen, Quanjiang Ji
Journal Article
Genome-edited crops: how to move them from laboratory to market
Kunling CHEN, Caixia GAO
Journal Article
A brief review of genome editing technology for generating animal models
Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH
Journal Article
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
Journal Article
is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice
Yuqin Men, Xiujuan Li, Hailong Tu, Aizhen Zhang, Xiaolong Fu, Zhishuo Wang, Yecheng Jin, Congzhe Hou, Tingting Zhang, Sen Zhang, Yichen Zhou, Boqin Li, Jianfeng Li, Xiaoyang Sun, Haibo Wang, Jiangang Gao
Journal Article